Determination of cholesterol and four phytosterols in foods without derivatization by gas chromatography-tandem mass spectrometry

In this study, a method for determination of cholesterol and four phytosterols by gas

chromatography coupled with electron impact ionization modeetandem mass spectrometry

without derivatization in general food was developed. The sample was saponified with

7.5% KOH in methanol. After heating on hot plate and reflux for 60 minutes, the saponified

portion was extracted with n-hexane/petroleum ether (50:50, v/v). The extracts were

evaporated with rotary evaporator and then redissolved with tetrahydrofuran. The tetrahydrofuran

layer was transferred into an injection vial and analyzed by gas chromatography

on a 30 m VF-5 column. Limit of quantification was 2 mg/kg. Recoveries of

cholesterol and four phytosterols from general food were between 91% and 100%.

※ author : Yan-Zong Chen, Shih-Yao Kao, Hao-Cheng Jian, Yu-Man Yu, Ju-Ying Li, Wei-Hsien Wang, Chung-Wei Tsai*

※ publisher : (SCI) Journal of Food and Drug Analysis, 23, 636-644

Copyright © 2015, Food and Drug Administration, Taiwan. Published by Elsevier Taiwan

LLC. All rights reserved.