DETERMINATION OF SIX PHTHALATE FROM HIGH-FAT FOOD BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY/ELECTROSPRAY IONIZATION-TANDEM MASS SPECTROMETRY

A LC/MS/MS method has been validated for simultaneous quantification of diisononyl phthalate (DINP), di-n-octyl phthalate (DNOP), Di(2-ethylhexyl)phthalate (DEHP), diisodecyl phthalate (DIDP), benzyl butyl phthalate (BBP) and dibutyl phthalate (DBP) in food. The phthalate in high-fat food were extracted into methanol, and diluted to 50 times. In this study, matrix effect on mass spectrometry response was investigated with available electrospray ionization (ESI). Typical recoveries of the analytes in soybean oil and butter were 31% to 83% and 22% to 84% at a fortification level 1.0 mg/kg, respectively. Samples after clean up with gel permeation chromatography (GPC) appeared to be less liable to matrix effect than samples without clean up. The recoveries in butter samples were in the ranges from 70% to 106%.

※作者author : Chung Wei Tsai*, Kuen Jou Wei, Yu Man Yu, Cheng Hung Huang, Ju Ying Li

※ 出版商publisher : 100年度食品衛生檢驗科技暨檢驗技術之挑戰-發現非法食品添加物